H4/n [ac Lys16] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35924

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-35924-100ul
NBP3-35924-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all H4/n Antibodies »

Product Specifications

Immunogen

A synthetic acetylated peptide around K16 of human H4/n (NP_003539.1).

Sequence:
GAKRH

Modification

ac Lys 16

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H4/n [ac Lys16] Antibody

H4/n [ac Lys16] Antibody

Western Blot: H4/n [ac Lys16] Antibody [NBP3-35924] -

Western Blot: H4/n [ac Lys16] Antibody [NBP3-35924] - Western blot analysis of lysates from NIH/3T3 cells, using H4/n Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37C for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for H4/n [ac Lys16] Antibody

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: H4/n

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. H4/N is a103 amino acid long, 11 kDA protein encoded by the HIST2H4A gene found on chromosome 1. HIST2H4A is one of four histone genes in this specific cluster that are duplicated, representing the centromeric copy. HIST2H4A is involved in type II interferon signaling, development notch signaling pathway, transcription Sin3 and NuRD in transcription regulation, and telomere maintenance through interactions with genes HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, and HIST1H3F. It has been investigated for its role in lymphoma, carcinoma, leukemia, herpes simplex, thyroiditis, and lupus.

Alternate Names

FO108, H4, H4 histone family, member N, H4 histone, family 2, H4/A, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/n, H4/O, H4F2H4/B, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FNH4/C, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4B, HIST4H4, histone 2, H4, histone 2, H4a, histone cluster 2, H4a, histone H4, histone IV, family 2

Gene Symbol

HIST2H4A

Additional H4/n Products

Product Documents for H4/n [ac Lys16] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for H4/n [ac Lys16] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.