HAND1 Antibody (OTI1G10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00576
![Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]](https://resources.bio-techne.com/images/products/HAND1-Antibody-1G10-Western-Blot-NBP2-00576-img0003.jpg "Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]")
![Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]](https://resources.bio-techne.com/images/products/HAND1-Antibody-1G10-Flow-Cytometry-NBP2-00576-img0001.jpg "Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]")
![Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]](https://resources.bio-techne.com/images/products/HAND1-Antibody-1G10-Western-Blot-NBP2-00576-img0005.jpg "Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, Western Blot
Cited:
In vitro assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1G10
Concentration
0.82 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HAND1 Antibody (OTI1G10)
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).![Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576]](https://resources.bio-techne.com/images/products/HAND1-Antibody-1G10-Immunocytochemistry-Immunofluorescence-NBP2-00576-img0004.jpg "Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576]")
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576]
Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.![Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]](https://resources.bio-techne.com/images/products/HAND1-Antibody-1G10-Flow-Cytometry-NBP2-00576-img0002.jpg "Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]")
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for HAND1 Antibody (OTI1G10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
In vitro assay
Reported in scientific literature (PMID:33316598)
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HAND1
Long Name
Heart And Neural Crest Derivatives Expressed 1
Alternate Names
eHand, Thing1
Entrez Gene IDs
9421 (Human)
Gene Symbol
HAND1
Additional HAND1 Products
Product Documents for HAND1 Antibody (OTI1G10)
Product Specific Notices for HAND1 Antibody (OTI1G10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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