HAND1 Antibody (OTI1G10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00576

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, Western Blot

Cited:

In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G10

Concentration

0.82 mg/ml

View all HAND1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HAND1 Antibody (OTI1G10)

Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]

Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576]

Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576]

Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576]

Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]

Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576]

Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
View 2 more images

Applications for HAND1 Antibody (OTI1G10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

In vitro assay

Reported in scientific literature (PMID:33316598)

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HAND1

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq].

Long Name

Heart And Neural Crest Derivatives Expressed 1

Alternate Names

eHand, Thing1

Entrez Gene IDs

9421 (Human)

Gene Symbol

HAND1

Additional HAND1 Products

Product Documents for HAND1 Antibody (OTI1G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HAND1 Antibody (OTI1G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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