HAND1 Antibody (OTI1G10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71459

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HAND1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HAND1 Antibody (OTI1G10) - Azide and BSA Free

Western Blot: HAND1 Antibody (OTI1G10)Azide and BSA Free [NBP2-71459]

Western Blot: HAND1 Antibody (OTI1G10)Azide and BSA Free [NBP2-71459]

Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459]

Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459]

Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459]

Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459]

Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for HAND1 Antibody (OTI1G10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HAND1

HAND1 is encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq]

Long Name

Heart And Neural Crest Derivatives Expressed 1

Alternate Names

eHand, Thing1

Gene Symbol

HAND1

Additional HAND1 Products

Product Documents for HAND1 Antibody (OTI1G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HAND1 Antibody (OTI1G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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