HDAC5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22152

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Histone Deacetylase 5/HDAC5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]

Reactivity Notes

Rat reactivity reported in scientific literature (Kim et al).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC5 Antibody - BSA Free

Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunohistochemistry: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.
View 1 more images

Applications for HDAC5 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 32196931)

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1.0 - 2.0 ug/ml
Application Notes
In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP2-22152 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 5/HDAC5

HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).

Long Name

Histone Deacetylase 5

Alternate Names

FLJ90614, KIAA0600, NY-CO-9

Entrez Gene IDs

10014 (Human)

Gene Symbol

HDAC5

UniProt

Q9UQL6 (Human)

Additional Histone Deacetylase 5/HDAC5 Products

Product Documents for HDAC5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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