HDAC5 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22152CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-22152AF350

Alexa Fluor 405

NBP2-22152AF405

Alexa Fluor 488

NBP2-22152AF488

Alexa Fluor 532

NBP2-22152AF532

Alexa Fluor 594

NBP2-22152AF594

Alexa Fluor 647

NBP2-22152AF647

Alexa Fluor 700

NBP2-22152AF700

Alexa Fluor 750

NBP2-22152AF750

Allophycocyanin

NBP2-22152APC

Biotin

NBP2-22152B

DyLight 350

NBP2-22152UV

DyLight 405

NBP2-22152V

DyLight 488

NBP2-22152G

DyLight 550

NBP2-22152R

DyLight 594

NBP2-22152DL594

DyLight 650

NBP2-22152C

DyLight 680

NBP2-22152FR

DyLight 755

NBP2-22152IR

FITC

NBP2-22152F

HRP

NBP2-22152H

Janelia Fluor 525

NBP2-22152JF525

Janelia Fluor 549

NBP2-22152JF549

Janelia Fluor 646

NBP2-22152JF646

Janelia Fluor 669

NBP2-22152JF669

mFluor Violet 450 SE

NBP2-22152MFV450

mFluor Violet 500 SE

NBP2-22152MFV500

mFluor Violet 610 SE

NBP2-22152MFV610

PE

NBP2-22152PE

PerCP

NBP2-22152PCP

Forumulation

Catalog #

BSA Free

NBP2-22152

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 5/HDAC5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]

Reactivity Notes

Rat reactivity reported in scientific literature (Kim et al).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for HDAC5 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Histone Deacetylase 5/HDAC5

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF(p300/CBP- associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Long Name

Histone Deacetylase 5

Alternate Names

FLJ90614, KIAA0600, NY-CO-9

Gene Symbol

HDAC5

Additional Histone Deacetylase 5/HDAC5 Products

Product Documents for HDAC5 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HDAC5 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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