Helicobacter Pylori Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29479
![Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]](https://resources.bio-techne.com/images/products/Helicobacter-Pylori-Antibody-Immunohistochemistry-Paraffin-NBP2-29479-img0002.jpg "Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]")
Key Product Details
Species Reactivity
H. pylori
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Total sonicate of Helicobacter pylori
Reactivity Notes
Helicobacter pylori.
Localization
Cell surface and cytoplasm
Specificity
The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33100)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Helicobacter Pylori Antibody
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.![Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]](https://resources.bio-techne.com/images/products/Helicobacter-Pylori-Antibody-Immunohistochemistry-Paraffin-NBP2-29479-img0001.jpg "Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]")
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Both methods allow for suitable detection, however using EDTA is the superior method.Applications for Helicobacter Pylori Antibody
Application
Recommended Usage
Immunohistochemistry
1-2ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Helicobacter Pylori
There is also strong evidence that Helicobacter pylori plays a causal role in the development of some stomach cancers, likely through chronic infection. However, Helicobacter pylori is also thought to play a role in normal stomach homeostasis which could help explain the large percent of people who have some level of Helicobacter pylori in their gut. The antibody can be used as an ancillary tool for identifying Helicobacter pylori by immunohistochemistry of tissue sections and may help determine if disease is linked to active infection. The antibody can also be used to study the presence of Helicobacter pylori in healthy tissue and may be a useful biomarker for further understanding the role of microbes in stomach homeostasis.
Alternate Names
H. pylori
Additional Helicobacter Pylori Products
Product Documents for Helicobacter Pylori Antibody
Product Specific Notices for Helicobacter Pylori Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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