Helicobacter Pylori Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29479

Novus Biologicals, part of Bio-Techne
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NBP2-29479
NBP2-29479-20ug
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Key Product Details

Species Reactivity

Validated:

H. pylori

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Helicobacter Pylori Antibodies »

Product Summary for Helicobacter Pylori Antibody

Immunogen

Total sonicate of Helicobacter pylori

Reactivity Notes

Helicobacter pylori.

Localization

Cell surface and cytoplasm

Specificity

The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33100)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Helicobacter Pylori Antibody

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody [NBP2-29479] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Both methods allow for suitable detection, however using EDTA is the superior method.

Applications for Helicobacter Pylori Antibody

Application
Recommended Usage

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Helicobacter Pylori

Helicobacter pylori (H.pylori) is a gram-negative, microaerophil bacterium found in the stomach. Although Helicobacter pylori is present in the upper gastrointestinal track of more than 50% of the world's population, about 80% of people infected with Helicobacter pylori are asymptomatic. However, Helicobacter pylori is also strongly linked to disease and is thought to have evolved to penetrate the mucoid lining of the stomach. Penetrating the mucosal layer can cause considerable damage to mucosa, and chronic infection can result in or contribute to chronic gastritis, gastric ulcers as well as duodenal ulcers.

There is also strong evidence that Helicobacter pylori plays a causal role in the development of some stomach cancers, likely through chronic infection. However, Helicobacter pylori is also thought to play a role in normal stomach homeostasis which could help explain the large percent of people who have some level of Helicobacter pylori in their gut. The antibody can be used as an ancillary tool for identifying Helicobacter pylori by immunohistochemistry of tissue sections and may help determine if disease is linked to active infection. The antibody can also be used to study the presence of Helicobacter pylori in healthy tissue and may be a useful biomarker for further understanding the role of microbes in stomach homeostasis.

Alternate Names

H. pylori

Additional Helicobacter Pylori Products

Product Documents for Helicobacter Pylori Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Helicobacter Pylori Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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