Helicobacter Pylori Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33100
Key Product Details
Species Reactivity
Validated:
H. pylori
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Helicobacter Pylori Antibody - Azide and BSA Free
Immunogen
Total sonicate of Helicobacter pylori
Reactivity Notes
Helicobacter pylori.
Localization
Cell surface and cytoplasm
Specificity
The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-29479).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Helicobacter Pylori Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0Applications for Helicobacter Pylori Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Helicobacter Pylori
Alternate Names
H. pylori
Additional Helicobacter Pylori Products
Product Documents for Helicobacter Pylori Antibody - Azide and BSA Free
Product Specific Notices for Helicobacter Pylori Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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