Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 22F6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Helios Antibodies »

Product Summary for Helios Antibody (22F6) [mFluor Violet 450 SE]

Immunogen

Peptide coresponding to the amino acids 51-107 of Helios.

Specificity

The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for Helios Antibody (22F6) [mFluor Violet 450 SE]

Helios Antibody (22F6) [mFluor Violet 450 SE] [NBP2-37723MFV450] -

Helios Antibody (22F6) [mFluor Violet 450 SE] [NBP2-37723MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for Helios Antibody (22F6) [mFluor Violet 450 SE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Helios

HELIOS encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Gene Symbol

IKZF2

Additional Helios Products

Product Documents for Helios Antibody (22F6) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Helios Antibody (22F6) [mFluor Violet 450 SE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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