His Tag Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM050
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Bovine, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Yeast
Applications
Validated:
Western Blot
Cited:
Binding Assay, Bioassay, ELISA Capture, ELISA Detection, ELISA Development, Epitope Mapping, Immunohistochemistry-Frozen
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Product Specifications
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. This antibody detects His tags localized at the amino- or carboxyl-terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag Biotinylated Antibody
Detection of His Tag by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for His Tag Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: HEK293T human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Sample: HEK293T human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 4.2 using BAM050 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Biotinylated Antibody
Product Specific Notices for His Tag Biotinylated Antibody
For research use only
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