Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Bacteria - Mycobacterium smegmatis, Bacteria - Streptococcus suis, Bovine, E. coli, Guinea Pig, Hamster, Insect, N/A, Primate, Virus, Yeast

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunoaffinity Purification, Simple Western, Western Blot

Cited:

Affinity Purification, Binding Assay, Bioassay, Co-IP, Cross-linking, ELISA (Capture), ELISA (detection), ELISA Development, ELISA Development (Capture), Epitope Mapping, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Knockdown, Neutralization, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10

View all His Tag Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for His Tag Antibody

Detection of His Tag antibody by Western Blot.

Detection of His Tag by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry.

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody.

Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (IC050A, filled histogram). No staining was observed in the absence of protein (open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.
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Applications for His Tag Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged proteins and SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line

Immunoaffinity Purification

Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.

Simple Western

10 µg/mL
Sample: CHO Chinese hamster ovary cell line transfected with His-tagged Human S1P4/EDG-6

Western Blot

0.2 µg/mL
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 14 reviews rated 4.8 using MAB050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for His Tag Antibody

For research use only

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