Histone H1.3 [p Thr17] Antibody (3K5C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16927

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16927-100ul
NBP3-16927-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3K5C10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H1.3 Antibodies »

Product Summary for Histone H1.3 [p Thr17] Antibody (3K5C10)

Immunogen

A synthetic phosphorylated peptide around T17 of human Histone H1.3 (NP_005311.1). EKTPV

Modification

p Thr 17

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H1.3 [p Thr17] Antibody (3K5C10)

Western Blot: Histone H1.3 [p Thr17] Antibody (3K5C10) [NBP3-16927] - Western blot analysis of extracts of various cell lines, using Histone H1.3 Rabbit mAb (NBP3-16927) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Histone H1.3 [p Thr17] Antibody (3K5C10)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H1.3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The histone H1.3 protein is encoded by the HIST1H1D gene and is 221 amino acids long at 22 kDA. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1D participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification and is known to interact with genes LMNA, EP300, SUMO1, NOP56 and YWHAQ. Researchers have investigated its role in treacher collins syndrome, meningioma, typhoid fever, and crohn's disease.

Alternate Names

H1 histone family, member 3, H1.3, H1F3H1D, histone 1, H1d, histone cluster 1, H1d, histone H1.3, Histone H1c, MGC138176

Gene Symbol

H1-3

Additional Histone H1.3 Products

Product Documents for Histone H1.3 [p Thr17] Antibody (3K5C10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H1.3 [p Thr17] Antibody (3K5C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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