Histone H2AX Antibody (4Z3S8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15378

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15378-100ul
NBP3-15378-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Z3S8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2AX Antibodies »

Product Summary for Histone H2AX Antibody (4Z3S8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2AX (P16104). MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX Antibody (4Z3S8)

Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Western Blot: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Western blot analysis of extracts of various cell lines, using Histone H2AX Rabbit mAb (NBP3-15378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Mouse brain using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (4Z3S8) [NBP3-15378] - Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H2AX antibody (NBP3-15378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Histone H2AX Antibody (4Z3S8)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:50-1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (4Z3S8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2AX Antibody (4Z3S8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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