Histone H2AX [p Ser139] Antibody (9F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19255

Novus Biologicals, part of Bio-Techne
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NBP1-19255
NBP1-19255-0.2mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Guinea Pig, Hamster, Primate, Rabbit, Sheep

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

This Histone H2AX [p Ser139] Antibody (9F3) was developed against a synthetic phospho-peptide derived from the sequence of human Histone H2AX phosphorylated at Ser139.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Modification

p Ser139

Specificity

Specific to the portion of Human Histone H2AX phosphorylated at Ser139.

Marker

DNA Double-strand break marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Scientific Data Images for Histone H2AX [p Ser139] Antibody (9F3)

Western Blot: Histone H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Analysis of Histone H2AX [p Ser139] antibody (9F3) Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate. Bands appear at an observed molecular weight of ~15 kDa.

Applications for Histone H2AX [p Ser139] Antibody (9F3)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry reported in scientific literature (PMID:33088425).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2AX

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

Alternate Names

H2AFX

Entrez Gene IDs

3014 (Human); 15270 (Mouse); 500987 (Rat)

Gene Symbol

H2AX

UniProt

P16104

Additional Histone H2AX Products

Product Documents for Histone H2AX [p Ser139] Antibody (9F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2AX [p Ser139] Antibody (9F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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