Histone H2AX [p Ser139] Antibody (BLR053F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14678

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14678
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR053F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2AX Antibodies »

Product Summary for Histone H2AX [p Ser139] Antibody (BLR053F)

Immunogen

surrounding Serine 140

Modification

p Ser139

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Histone H2AX [p Ser139] Antibody (BLR053F)

Western Blot: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Western Blot: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human HeLa cells treated with etoposide. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.
Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human breast carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:100. Secondary: DyLight(R) 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.
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Applications for Histone H2AX [p Ser139] Antibody (BLR053F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/1mg lysate

Western Blot

1:1000
Application Notes
Flow-Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX [p Ser139] Antibody (BLR053F)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2AX [p Ser139] Antibody (BLR053F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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