Histone H2AX Antibody (CL5157)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21157

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL5157

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histone H2AX Antibodies »

Product Summary for Histone H2AX Antibody (CL5157)

Immunogen

This antibody was developed against a Synthetic Peptide of P16104. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Histone H2AX Antibody (CL5157)

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -

Staining of MCF7 cells using the Anti-H2AFX monoclonal antibody, showing specific staining in nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -

Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -

Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.
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Applications for Histone H2AX Antibody (CL5157)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 - 1:50000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (CL5157)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX Antibody (CL5157)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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