Histone H2AX Antibody (S02-3C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20068

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20068-100ul
NBP3-20068-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-3C8

Concentration

0.3 mg/ml

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Histone H2AX (Uniprot # P16104)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H2AX Antibody (S02-3C8)

Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068]

Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068]

Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Western blot detection of Histone H2AX in Hela, Ramos using NBP3-20068 (1:1000 diluted)
Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068]

Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Immunohistochemistry of Histone H2AX in paraffin-embedded Human lung cancer tissue using NBP3-20068 at dilution 1/50

Applications for Histone H2AX Antibody (S02-3C8)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (S02-3C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX Antibody (S02-3C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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