Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).

Specificity

This is specific for H2AX. It is not known if this also recognizes the phosphorylated form of H2AX.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX Antibody

Western Blot: Histone H2AX Antibody [NB100-383]

Western Blot: Histone H2AX Antibody [NB100-383]

Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB
Western Blot: Histone H2AX Antibody [NB100-383]

Western Blot: Histone H2AX Antibody [NB100-383]

Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Applications for Histone H2AX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-1:1000

Immunohistochemistry-Paraffin

1:500-1:2000

Simple Western

10 ug/ml

Western Blot

1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes a band at ~25 kDa, representing monoubiquinated H2AX protein and ~14 kDa, representing H2AX. IP, IHC reported in (PMID: 22426396). ICC/IF reported in (PMID: 20203688). br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Histone H2AX

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

Alternate Names

H2AFX

Entrez Gene IDs

3014 (Human); 15270 (Mouse)

Gene Symbol

H2AX

UniProt

P16104

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H2AX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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