Histone H2AX Antibody

Western Blot: Histone H2AX Antibody [NB100-638]

Western Blot: Histone H2AX Antibody [NB100-638] - Downregulation of the transcription factors Slug and ZEB1 reverses the EMT programme induced by H2A.X deficiency. Control cells (shCTRL) and cells silenced for H2A.X (shH2A.X) were transfected for 3 days with control siRNA (siC) or with a pool of siSLUG and siZEB1 (siS/Z). Immunoblot analysis of EMT markers was performed using tubulin as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/ncomms10711), licensed under a CC-BY license.

Western Blot: Histone H2AX Antibody [NB100-638]

Western Blot: Histone H2AX Antibody [NB100-638] - H2A.X re-expression inhibits EMT and promotes metastatic colonization in the lung. Immunoblot analysis of EMT markers in HCT116 parental cells (WT), H2A.X knockout cells (KO) and H2A.X knockout cells in which H2A.X expression was restored (KO+H2A.X), utilizing actin as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/ncomms10711), licensed under a CC-BY license.

Western Blot: Histone H2AX Antibody [NB100-638] -

Western Blot: Histone H2AX Antibody [NB100-638] - Knockdown of CAPER induces DSB proteins ATM & H2AX & leads to apoptosis in MDA-MB-231 cells(A) H2AX phosphorylation on ser139 is significantly increased (upper right quadrant population) in MDA-MB-231 cells after CAPER knockdown as represented by Muse Cell Analyzer plots (3-fold, p < 0.001, n = 3). (B) The increase in gammaH2AX was also validated through western blot analysis (2.5-fold, p < 0.01, n = 3). ATM phosphorylation on serine1981 is significantly upregulated after knockdown of CAPER expression (5-fold, p < 0.05, n = 3). (C) CAPER knockdown resulted in an increased level of caspase-3/7 activation through decreasing live cells (1.2-fold, p < 0.001, n = 3), while increasing apoptotic (7.5-fold, p < 0.001, n = 3), apoptotic/dead (6.5-fold, p < 0.001, n = 3), & dead (3-fold, p < 0.05, n = 3) cell populations. Interestingly, MDA-MB-231 cells expressing CAPER knockdown displayed no significant changes in any of the phases of the cell cycle (p = NS, n = 8, for G1, S & G2/M phases) compared to CTL shRNA (Figure 7C right panels). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30100993), licensed under a CC-BY license. Not internally tested by Novus Biologicals.