Histone H2AX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27428

Novus Biologicals, part of Bio-Techne
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NBP2-27428
NBP2-27428SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

A portion of amino acids 50-110 of human Histone H2A.x was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX Antibody

Western Blot: Histone H2AX Antibody [NBP2-27428]

Western Blot: Histone H2AX Antibody [NBP2-27428]

Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody [NBP2-27428]

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody [NBP2-27428]

Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP2-27428]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP2-27428]

Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27428] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.
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Applications for Histone H2AX Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10ug/ml

Simple Western

reported by internal validation

Western Blot

4-7ug/ml~
Application Notes
In Simple Western internal validation: Transfected HeLa, Human Primary Fibroblast Lysates as samples; separated by size; antibody dilution of 1:100; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AX

H2A.x is a human histone H2A isoprotein containing 143 amino acid residues. H2A.x is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of H2A.x mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. H2A.x becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites. It is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. It maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Alternate Names

H2AFX

Gene Symbol

H2AX

UniProt

P16104

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2AX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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