HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-405

Novus Biologicals, part of Bio-Techne
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NB500-405
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # HL-37

Format

BSA Free

Concentration

1 mg/ml

View all HLA DQ1/DQ3 Antibodies »

Product Summary for HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

Immunogen

Burkitt's lymphoma cell line Raji

Specificity

The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

Flow Cytometry: HLA DQ1/DQ3 Antibody (HL-37) - BSA Free [NB500-405]

Flow Cytometry: HLA DQ1/DQ3 Antibody (HL-37) - BSA Free [NB500-405]

Flow Cytometry: HLA DQ1/DQ3 Antibody (HL-37) [NB500-405] - Surface staining of human peripheral blood cells with anti-HLA-DQ1+DQ3 (HL-37) purified, GAM-APC

Applications for HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot: use non-reducing conditions. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA DQ1/DQ3

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of analpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system bypresenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells(APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons.Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes thetransmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the betachain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 differentmolecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. (provided by RefSeq)

Alternate Names

CELIAC1, HLA class II histocompatibility antigen, DQ beta 1 chain, HLA-DQB, IDDM1, MHC class II antigen DQB1, MHC class II antigen HLA-DQ-beta-1, MHC class II DQ beta chain, MHC class II HLA-DQ beta glycoprotein

Gene Symbol

HLA-DQA1

Additional HLA DQ1/DQ3 Products

Product Documents for HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DQ1/DQ3 Antibody (HL-37) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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