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HLA DQ1/DQ3 Antibody (HL-37) [Alexa Fluor® 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-405AF405

Novus Biologicals, part of Bio-Techne
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NB500-405AF405

Key Product Details

Species Reactivity

Human, Porcine

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # HL-37

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Burkitt's lymphoma cell line Raji

Reactivity Notes

Cross-reacts with Human.

Specificity

The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for HLA DQ1/DQ3 Antibody (HL-37) [Alexa Fluor® 405]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HLA DQ1/DQ3

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of analpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system bypresenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells(APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons.Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes thetransmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the betachain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 differentmolecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. (provided by RefSeq)

Alternate Names

CELIAC1, HLA class II histocompatibility antigen, DQ beta 1 chain, HLA-DQB, IDDM1, MHC class II antigen DQB1, MHC class II antigen HLA-DQ-beta-1, MHC class II DQ beta chain, MHC class II HLA-DQ beta glycoprotein

Gene Symbol

HLA-DQA1

Additional HLA DQ1/DQ3 Products

Product Documents for HLA DQ1/DQ3 Antibody (HL-37) [Alexa Fluor® 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA DQ1/DQ3 Antibody (HL-37) [Alexa Fluor® 405]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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