HLA-E Antibody (MEM-E/07) [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-310G
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-E/07
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Localization
Type I membrane protein.
Specificity
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for HLA-E Antibody (MEM-E/07) [DyLight 488]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: HLA-E
Long Name
Major Histocompatibility Complex, Class I, E
Alternate Names
HLA-6.2, HLAE, QA1
Gene Symbol
HLA-E
Additional HLA-E Products
Product Documents for HLA-E Antibody (MEM-E/07) [DyLight 488]
Product Specific Notices for HLA-E Antibody (MEM-E/07) [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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