HLA-E Antibody (MEM-E/07) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-310PECY55
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-E/07
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HLA-E Antibody (MEM-E/07) [PE/Cy5.5]
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Localization
Type I membrane protein.
Specificity
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for HLA-E Antibody (MEM-E/07) [PE/Cy5.5]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: HLA-E
Long Name
Major Histocompatibility Complex, Class I, E
Alternate Names
HLA-6.2, HLAE, QA1
Gene Symbol
HLA-E
Additional HLA-E Products
Product Documents for HLA-E Antibody (MEM-E/07) [PE/Cy5.5]
Product Specific Notices for HLA-E Antibody (MEM-E/07) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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