Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 245106

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HM74A/PUMA-G/GPR109A Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human HM74A/GPR109A
Met1-Pro363
Accession # Q8TDS4

Specificity

Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: HM74A/PUMA-G/GPR109A

Nicotinic acid receptors are a group of Gi/Go protein-coupled receptors that are currently divided into GPR81, GPR109Aand GPR109B subtypes. GPR109B receptors originated from a gene duplication of GPR109A and have > 95% homology. Allsubtypes are widely expressed throughout adipose tissue and are found at lower levels on macrophages, neutrophils,Langerhans cells and in the spleen. Nicotinic acid receptors are involved in the inhibition of lipolysis. The humangenes encoding these receptors are located on chromosome 12 (12q24.3)

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Alternate Names

GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG

Gene Symbol

HCAR2

Additional HM74A/PUMA-G/GPR109A Products

Product Documents for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [PE/Cy5.5]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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