Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (100%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19N10B7

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 protein

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react with Rat (99%), Bovine (99%), Porcine/Pig (99%), Chinese Hamster (99%), Chicken (91%), Canine/Dog (100%), Primate/Monkey (100%) and Equine/Horse (100%).

Localization

Nucleus (very high), Cytoplasm (very low)

Specificity

This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)Azide and BSA Free [NBP2-27395]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)Azide and BSA Free [NBP2-27395]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7) - Azide Free [NBP2-27395] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free [NBP2-27395]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free [NBP2-27395]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - Azide Free [NBP2-27395] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human colon using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows specific HMGB1 nuclear positivity in different mucosal cells of the colon. Image using the standard format of this product.
Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free [NBP2-27395]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free [NBP2-27395]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - Azide Free [NBP2-27395] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). this antibody was used for this test.
View 13 more images

Applications for HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5ug/ml~

Western Blot

1-3ug/ml~

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 Antibody (19N10B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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