Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Simple Western, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19N12A1

Concentration

1.0 mg/ml

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 was used as the immunogen.

Specificity

This antibody recognizes both B box domain (amino acids 88-162) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N12A1)

Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400]

Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400]

Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400]

Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400]

Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Image shows a specific band for HMGB1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for HMGB1/HMG-1 Antibody (19N12A1)

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 29601597)

Simple Western

1:100

Western Blot

1 - 3 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

UniProt

P09429

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N12A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HMGB1/HMG-1 Antibody (19N12A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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