HMGB1/HMG-1 Antibody (19N15F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27401

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (99%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 19N15F4

Concentration

1.0 mg/ml

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 was used as the immunogen for the antibody.

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react 100% with olive baboon, crab-eating macaque, and common marmoset.

Localization

Nucleus (very high, ubiquitous), Cytoplasmic (very low, some tissues)

Specificity

This antibody recognizes both the box A domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N15F4)

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein on ( A) Full-length human HMGB1 protein (B) human Jurkat cell lysate and (C) mouse NIH 3T3 cell lysate using HMGB1 antibody (clone 19N15F4) at 2 ug/ml concentration. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used in this assay.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - IHC-P detection of HMGB1 protein in formalin-fixed, decalcified, paraffin embedded tissue sections from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml [Image courtesy of Dr Ulf Andersson, Karolinska]
Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).
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Applications for HMGB1/HMG-1 Antibody (19N15F4)

Application
Recommended Usage

Flow Cytometry

0.5ug/5x10^5cells~

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

1-3ug/ml~

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N15F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 Antibody (19N15F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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