HMGB1/HMG-1 Antibody (1A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15235

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HMGB1/HMG-1. UniProt ID (P09429).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGB1/HMG-1 Antibody (1A1)

Western Blot: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235]

Western Blot: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235]

Western Blot: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate. All lanes: HMGB1/HMG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 KDa. Observed band size: 25 KDa.
Immunohistochemistry: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235]

Immunohistochemistry: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235]

Immunohistochemistry: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235] - IHC image of HMGB1/HMG-1 antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for HMGB1/HMG-1 Antibody (1A1)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (1A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 Antibody (1A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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