HNF-4 alpha/NR2A1 Antibody (OTI2H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45989

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H2

Concentration

1 mg/ml

View all HNF-4 alpha/NR2A1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-315 of human HNF4A(NP_849180) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (OTI2H2)

Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) [NBP2-45989]

Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) [NBP2-45989]

Western Blot: HNF-4 alpha/NR2A1 Antibody (2H2) [NBP2-45989] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF-4 alpha.
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) [NBP2-45989]

Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) [NBP2-45989]

Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (2H2) [NBP2-45989] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Applications for HNF-4 alpha/NR2A1 Antibody (OTI2H2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody (OTI2H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (OTI2H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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