Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HNF-4 alpha/NR2A1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-315 of human HNF4A(NP_849180) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2)Azide and BSA Free [NBP2-70905]

Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2)Azide and BSA Free [NBP2-70905]

Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF-4 alpha.
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905]

Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905]

Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Applications for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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