HO-2/HMOX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36745

Novus Biologicals, part of Bio-Techne
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NBP1-36745
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all HO-2/HMOX2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DQAGSTLARETLED corresponding to internal region according to NP_002125.3.

Epitope

internal region

Specificity

Reported variants represent identical protein: NP_002125.3, NP_001120676.1, NP_001120678.1, NP_001120677.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HO-2/HMOX2 Antibody

Western Blot: HO-2/HMOX2 Antibody [NBP1-36745]

Western Blot: HO-2/HMOX2 Antibody [NBP1-36745]

Western Blot: HO-2/HMOX2 Antibody [NBP1-36745] - Analysis of K562 cell lysate (35ug protein in RIPA buffer) using NBP1-36745 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for HO-2/HMOX2 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in K562 lysates (calculated MW of 36.0 kDa band according to NP_002125.3). IHC-P: Human pancreas cancer shows increased staining of invading cancer cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HO-2/HMOX2

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus.

Long Name

Heme Oxygenase 2

Alternate Names

HMOX2, HO2

Entrez Gene IDs

3163 (Human); 15369 (Mouse)

Gene Symbol

HMOX2

UniProt

NP_002125.3 (Human)

Additional HO-2/HMOX2 Products

Product Documents for HO-2/HMOX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HO-2/HMOX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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