Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Hrk Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk. Amino Acid Squence: MWRRRARSRRAPAPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hrk Antibody - BSA Free

Western Blot: Hrk AntibodyBSA Free [NBP1-76414]

Western Blot: Hrk AntibodyBSA Free [NBP1-76414]

Western Blot: Hrk Antibody [NBP1-76414] - Mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 u/mL.

Applications for Hrk Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

2.5-5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hrk

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.

Long Name

Harakiri

Alternate Names

activator of apoptosis harakiri, activator of apoptosis Hrk, BCL2-interacting protein, BH3-interacting domain-containing protein 3, BID3, death protein 5, DP5, HARAKIRI, harakiri, BCL2 interacting protein (contains only BH3 domain), harakiri, BCL2-interacting protein (contains only BH3 domain), Neuronal death protein DP5

Gene Symbol

HRK

UniProt

NP_003797 (Human)

Additional Hrk Products

Product Documents for Hrk Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hrk Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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