HSD3B1 Antibody (FDO66Q)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-78644

Novus Biologicals, part of Bio-Techne
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NB110-78644
NB110-78644SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Goat, Primate, Sheep

Cited:

Mouse, Rat, Porcine, Bovine, Goat, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FDO66Q

Concentration

1 mg/ml

View all HSD3B1 Antibodies »

Product Specifications

Immunogen

JEG Choriocarcinoma cells

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 31158446). Porcine reactivity reported in scientific literature (Bidne et al). Sheep reactivity reported in scientific literature (PMID: 32932187). Use in Mouse reported in scientific literature (PMID:32592754).

Localization

Membrane

Marker

Trophoblast-associated Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSD3B1 Antibody (FDO66Q)

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644]

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644]

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.

Applications for HSD3B1 Antibody (FDO66Q)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Immunohistochemistry

10-20 ug/ml

Immunohistochemistry-Frozen

10-20 ug/ml

Immunohistochemistry-Paraffin

10-20 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSD3B1

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Alternate Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3BETAHSD, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, delta-5-3-ketosteroid isomerase, EC 1.1.1, HSD3B, HSDB3, HSDB3A, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1, I, progesterone reductase, SDR11E1, short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase, steroid Delta-isomerase, Trophoblast antigen FDO161G

Gene Symbol

HSD3B1

Additional HSD3B1 Products

Product Documents for HSD3B1 Antibody (FDO66Q)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSD3B1 Antibody (FDO66Q)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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