HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80781

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Goat, Primate, Sheep

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FDO66Q

Format

Azide and BSA Free

Concentration

1 mg/ml

View all HSD3B1 Antibodies »

Product Summary for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

Immunogen

JEG Choriocarcinoma cells

Reactivity Notes

Marmoset.

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) - Azide and BSA Free [NBP2-80781]

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) - Azide and BSA Free [NBP2-80781]

Immunohistochemistry: HSD3B1 Antibody (FDO66Q) - Azide and BSA Free [NBP2-80781] - Staining of placental villi. Image from the standard format of this antibody.

Applications for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Immunohistochemistry

10-20 ug/ml

Immunohistochemistry-Frozen

10-20 ug/ml

Immunohistochemistry-Paraffin

10-20 ug/ml
Application Notes
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSD3B1

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Alternate Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3BETAHSD, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, delta-5-3-ketosteroid isomerase, EC 1.1.1, HSD3B, HSDB3, HSDB3A, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1, I, progesterone reductase, SDR11E1, short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase, steroid Delta-isomerase, Trophoblast antigen FDO161G

Gene Symbol

HSD3B1

Additional HSD3B1 Products

Product Documents for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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