HSD3B1 Antibody (FDO66Q) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-78644F

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NB110-78644

Alexa Fluor 350

NB110-78644AF350

Alexa Fluor 405

NB110-78644AF405

Alexa Fluor 488

NB110-78644AF488

Alexa Fluor 532

NB110-78644AF532

Alexa Fluor 594

NB110-78644AF594

Alexa Fluor 647

NB110-78644AF647

Alexa Fluor 700

NB110-78644AF700

Alexa Fluor 750

NB110-78644AF750

Biotin

NB110-78644B

CoraFluor 1

NB110-78644CL1

DyLight 350

NB110-78644UV

DyLight 405

NB110-78644V

DyLight 488

NB110-78644G

DyLight 550

NB110-78644R

DyLight 594

NB110-78644DL594

DyLight 650

NB110-78644C

DyLight 680

NB110-78644FR

DyLight 755

NB110-78644IR

HRP

NB110-78644H

Janelia Fluor 525

NB110-78644JF525

Janelia Fluor 549

NB110-78644JF549

Janelia Fluor 585

NB110-78644JF585

Janelia Fluor 635

NB110-78644JF635

Janelia Fluor 646

NB110-78644JF646

Janelia Fluor 669

NB110-78644JF669

mFluor Violet 450 SE

NB110-78644MFV450

mFluor Violet 500 SE

NB110-78644MFV500

mFluor Violet 610 SE

NB110-78644MFV610

Forumulation

Catalog #

Azide and BSA Free

NBP2-80781

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Goat, Primate, Sheep

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # FDO66Q

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSD3B1 Antibodies »

Product Summary for HSD3B1 Antibody (FDO66Q) [FITC]

Immunogen

JEG Choriocarcinoma cells

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 31158446). Porcine reactivity reported in scientific literature (Bidne et al). Sheep reactivity reported in scientific literature (PMID: 32932187). Use in Mouse reported in scientific literature (PMID:32592754).

Localization

Membrane

Marker

Trophoblast-associated Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for HSD3B1 Antibody (FDO66Q) [FITC]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HSD3B1

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Alternate Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3BETAHSD, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, delta-5-3-ketosteroid isomerase, EC 1.1.1, HSD3B, HSDB3, HSDB3A, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1, I, progesterone reductase, SDR11E1, short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase, steroid Delta-isomerase, Trophoblast antigen FDO161G

Gene Symbol

HSD3B1

Additional HSD3B1 Products

Product Documents for HSD3B1 Antibody (FDO66Q) [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HSD3B1 Antibody (FDO66Q) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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