Human 4-1BB Ligand/TNFSF9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB22952

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB22952-100
MAB22952-SP
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2357A

View all 4-1BB Ligand/TNFSF9 Antibodies »

Product Specifications

Immunogen

E. coli-derived human 4-1BB Ligand/TNFSF9
Arg71-Glu254
Accession # P41273.1

Specificity

Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human 4-1BB Ligand/TNFSF9 Antibody

Detection of 4-1BB Ligand/TNFSF9 antibody in Human Daudi cell line antibody by Flow Cytometry.

Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry.

Human Daudi Burkitt lymphoma cell line was stained with Rabbit Anti-Human 4-1BB Ligand/TNFSF9 Mono-clonal Antibody (Catalog # MAB22952, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Applications for Human 4-1BB Ligand/TNFSF9 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human Daudi cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References

  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
  5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
  7. Baessler, T. et al. (2010) Blood 115:3058.
  8. Gullo, C. et al. (2010) PLoS One 5:e10845.
  9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
  10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  11. Wen, T. et al. (2002) J. Immunol. 168:4897.
  12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
  13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
  14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
  15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Gene Symbol

TNFSF9

UniProt

P41273.1

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for Human 4-1BB Ligand/TNFSF9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human 4-1BB Ligand/TNFSF9 Antibody

For research use only

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