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Rabbit IgG PE-conjugated Antibody Best Seller

R&D Systems, part of Bio-Techne | Catalog # F0110

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Rabbit

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Rabbit IgG

Specificity

Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG PE-conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (MAB6944) or (B) Normal Rabbit IgG Control (AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) at 10 µL/106 cells.

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

U937 cells were stained with Rabbit Anti-Human IFN alpha betaR1 Monoclonal Antibody (2452, filled histogram) or (B) Normal Rabbit IgG Control (AB-105-C, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) at 10 µL/106 cells.

Applications for Rabbit IgG PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using F0110 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rabbit IgG PE-conjugated Antibody

For research use only

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