Human 5-HT1D Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10354
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 1014728
Product Specifications
Immunogen
Synthetic peptide containing human 5-HT1D
Accession # P28221
Accession # P28221
Specificity
Detects human 5-HT1D in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human 5-HT1D Antibody
5-HT1D in Human Brain.
5-HT1D was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human 5-HT1D Monoclonal Antibody (Catalog # MAB10354) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of 5-HT1D in Rat Brain.
5-HT1D was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Mouse Anti-Human 5-HT1D Monoclonal Antibody (Catalog # MAB10354) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human 5-HT1D Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) tissue and perfusion fixed paraffin-embedded sections of Rat Brain
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) tissue and perfusion fixed paraffin-embedded sections of Rat Brain
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5-HT1D
Long Name
5-Hydroxytryptamine Receptor 1D
Alternate Names
5HT1D, HT1DA, HTR1D, HTR1DA, HTRL, RDC4
Gene Symbol
HTR1D
UniProt
Additional 5-HT1D Products
Product Documents for Human 5-HT1D Antibody
Product Specific Notices for Human 5-HT1D Antibody
For research use only
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