A172 human glioblastoma cell line was stained with Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

5HT2C in Human Brain.

5HT2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5HT2C Monoclonal Antibody (Catalog # MAB5764) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human 5-HT2C Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: A172 human glioblastoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT2C

5HT2C is a 45 kDa 7TM receptor for serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5HT2C ligation regulates mood, appetite, and motivation. 5HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5HT2C can be generated by RNA editing. Human 5HT2C shares 89% aa sequence identity with mouse and rat 5HT2C.

Long Name

5-Hydroxytryptamine Receptor 2C

Alternate Names

5-HTR2C, 5HT2C, HTR2C, Serotonin Receptor 2C

Entrez Gene IDs

3358 (Human); 15560 (Mouse); 25187 (Rat)

Gene Symbol

HTR2C

Additional 5-HT2C Products

Product Documents for Human 5-HT2C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 5-HT2C Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human 5-HT2C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5764

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all 5-HT2C Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 5-HT2C Antibody

Detection of 5HT2C in A172 Human Cell Line by Flow Cytometry.