HEK293 human embryonic kidney cell line transfected with human 5-HT2C and eGFP was stained with either (A) Mouse Anti-Human 5-HT2C APC-conjugated Monoclonal Antibody (Catalog # FAB5764A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human 5-HT2C APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human 5-HT2C and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5-HT2C

5-HT2C is a 45 kDa 7TM receptor that belongs to the G-protein coupled receptor #1 family. It binds serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5-HT2C ligation regulates mood, appetite, and motivation. 5-HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5-HT2C can be generated by RNA editing. Human 5-HT2C shares 89% aa sequence identity with mouse and rat 5-HT2C.

Long Name

5-Hydroxytryptamine Receptor 2C

Alternate Names

5-HTR2C, 5HT2C, HTR2C, Serotonin Receptor 2C

Entrez Gene IDs

3358 (Human); 15560 (Mouse); 25187 (Rat)

Gene Symbol

HTR2C

UniProt

P28335

Additional 5-HT2C Products

Product Documents for Human 5-HT2C APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 5-HT2C APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human 5-HT2C APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5764A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all 5-HT2C Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 5-HT2C APC-conjugated Antibody

Detection of 5‑HT2C in HEK293 Human Cell Line Transfected with Human 5-HT2C and eGFP by Flow Cytometry.