Human 53BP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB18772
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1285C
Product Specifications
Immunogen
E. coli-derived recombinant human 53BP1
Ala1614-His1972
Accession # Q12888
Ala1614-His1972
Accession # Q12888
Specificity
Detects human 53BP1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human 53BP1 Antibody
Detection of Human 53BP1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
53BP1 in HeLa Human Cell Line.
53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
53BP1 in Human Cervical Cancer Tissue.
53BP1 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human 53BP1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cervical epithelial carcinoma cell line
Sample: Immersion fixed paraffin-embedded sections of human cervical epithelial carcinoma cell line
Knockout Validated
53BP1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in 53BP1 knockout HeLa cell line.
Western Blot
2 µg/mL
Sample: HEK293 human embryonic kidney cell line and ZR‑75 human breast cancer cell line
Sample: HEK293 human embryonic kidney cell line and ZR‑75 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 53BP1
The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and therefore aids in nucleating a DNA damage response protein complex. Over aa 1614-1972, human 53BP1 shares 97% aa sequence identity with rat and mouse 53BP1.
Long Name
p53 Binding Protein 1
Alternate Names
p202, TP53BP1
Gene Symbol
TP53BP1
UniProt
Additional 53BP1 Products
Product Documents for Human 53BP1 Antibody
Product Specific Notices for Human 53BP1 Antibody
For research use only
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