Human Acetylated p53 (K382) Antibody

Detection of Human Acetylated p53 (K382) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with doxorubicin and trichostatin A (TSA). PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for acetylated p53 (K382) at approximately 53 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Acetylated p53 (K382) in Lung Cancer.

Acetylated p53 (K382) was detected in immersion fixed paraffin-embedded sections of lung cancer tissue using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Acetylated p53 (K382) in HeLa Human Cell Line.

Acetylated p53 (K382) was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with trichostatin A (TSA) and doxorubicin (left panel; positive staining) and untreated HeLa cell line (right panel; negative control) using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.