Human PBMC were stained with (A) Mouse Anti-Human ADAM28 Monoclonal Antibody (Catalog # MAB93252) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ADAM28 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3). ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha4 beta1(9). R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

References

Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
Howard, L. et al. (2000) Biochem. J. 348:21.
Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
Blobel, C.P. (1997) Cell 90:589.
Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

Long Name

A Disintegrin and Metalloproteinase Domain 28

Alternate Names

Dtgn1, eMDCII, MDC-Lm, MDC-Ls, MDCL, Protein-L

Entrez Gene IDs

10863 (Human); 13522 (Mouse); 290344 (Rat)

Gene Symbol

ADAM28

UniProt

Q0UKQ2

Additional ADAM28 Products

Product Documents for Human ADAM28 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ADAM28 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ADAM28 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB93252

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all ADAM28 Antibodies »

Product Summary for Human ADAM28 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ADAM28 Antibody

Detection of ADAM28 in Human PBMC by Flow Cytometry.