Human ADAM33 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5565
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunoprecipitation, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 508101
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM33
Val30-Leu501 (predicted)
Accession # Q9BZ11
Val30-Leu501 (predicted)
Accession # Q9BZ11
Specificity
Detects human ADAM33 in direct ELISAs. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse ADAM33 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ADAM33 Antibody
Detection of ADAM33 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti-Human ADAM33 Monoclonal Antibody (Catalog # MAB5565, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human ADAM33 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM33, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM33, see our available Western blot detection antibodies
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM33
ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.
Long Name
A Disintegrin and Metalloproteinase Domain 33
Alternate Names
a disintegrin and metalloprotease 33, a disintegrin and metalloproteinase domain 33, ADAM 33, ADAM metallopeptidase domain 33, C20orf153, chromosome 20 open reading frame 153, disintegrin and metalloproteinase domain-containing protein 33, disintegrin and reprolysin metalloproteinase family protein, dJ964F7.1, DKFZp434K0521, EC 3.4.24.-, FLJ35308, FLJ36751, MGC149823, MGC71889
Gene Symbol
ADAM33
UniProt
Additional ADAM33 Products
Product Documents for Human ADAM33 Antibody
Product Specific Notices for Human ADAM33 Antibody
For research use only
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