Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ADAMTS15 in Human Kidney.

ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ADAMTS15 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human ADAMTS15 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: MCF‑7 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB5149 in the following applications:

ELISA (2 Reviews)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS15

A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) is a secreted multi-domain protease that is primarily expressed in fetal liver and kidney. ADAMTS1, 4, 5, 8, and 15 form a subfamily of ADAMTS proteases that possess aggrecanase activity. These proteins are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. ADAMTS15 functions as a suppressor of tumor growth and invasion. It is downregulated in colon cancer, and its expression in breast cancer correlates with poor prognosis. Within amino acids (aa) 18-682 (which includes the propeptide, peptidase, disintegrin, first TSP-1 and most of the Cys-rich domain), human ADAMTS15 shares 95% aa sequence identity with mouse and rat ADAMTS15.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein, ADAM metallopeptidase with thrombospondin type 1 motif, 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, EC 3.4.24, EC 3.4.24.-, EC 3.4.24.14, EC 3.4.24.82, metalloprotease disintegrin 15 with thrombospondin domains, MGC126403

Entrez Gene IDs

170689 (Human); 235130 (Mouse); 300474 (Rat)

Gene Symbol

ADAMTS15

UniProt

Q8TE58

Additional ADAMTS15 Products

Product Documents for Human ADAMTS15 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ADAMTS15 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ADAMTS15 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5149

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all ADAMTS15 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ADAMTS15 Antibody

Detection of Human ADAMTS15 by Western Blot.