Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6414
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 739044
Product Specifications
Immunogen
E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
Glu2-Phe219
Accession # Q9UKK9
Glu2-Phe219
Accession # Q9UKK9
Specificity
Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Detection of Human ADP‑Sugar Pyrophosphatase/NUDT5 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Monoclonal Antibody (Catalog # MAB6414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADP-Sugar Pyrophosphatase/NUDT5
ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).
References
- McLennan, A.G. (2006) Cell Mol. Life Sci. 63:123.
- Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
- Zha, M. et al. (2008) J. Mol. Biol. 379:568.
- Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
- Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
- Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.
Alternate Names
Nudix motif 5, YSA1, YSA1H
Entrez Gene IDs
11164 (Human)
Gene Symbol
NUDT5
UniProt
Additional ADP-Sugar Pyrophosphatase/NUDT5 Products
Product Documents for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Product Specific Notices for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody
For research use only
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