Human Aggrecan (ADAMTS5-cleaved) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB64892
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 725913
Product Specifications
Immunogen
KLHG-conjugated human Aggrecan synthetic peptide
Leu384-Glu392
Accession # P16112
Leu384-Glu392
Accession # P16112
Specificity
Detects human Aggrecan in direct ELISAs. It specifically recognizes the N-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Aggrecan (ADAMTS5-cleaved) Antibody
Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot.
Western blot shows recombinant human Aggregan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan N-terminal fragment at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Aggrecan (ADAMTS5-cleaved) Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Recombinant human Aggregan N-terminal and C-terminal fragments
Sample: Recombinant human Aggregan N-terminal and C-terminal fragments
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aggrecan
Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases.
Alternate Names
ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK
Entrez Gene IDs
176 (Human)
Gene Symbol
ACAN
UniProt
Additional Aggrecan Products
Product Documents for Human Aggrecan (ADAMTS5-cleaved) Antibody
Product Specific Notices for Human Aggrecan (ADAMTS5-cleaved) Antibody
For research use only
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