Human AICL/CLEC-2B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9059

R&D Systems, part of Bio-Techne
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MAB9059-100
MAB9059-SP
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1064935

View all AICL/CLEC-2B Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B
Lys26-His149
Accession # Q92478

Specificity

Detects human AICL/CLEC-2B in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human AICL/CLEC-2B Antibody

Detection of AICL/CLEC-2B in U937 cells by Flow Cytometry.

U937 cells were stained with Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB9059, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human AICL/CLEC-2B Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AICL/CLEC-2B

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

References

  1. Li, Y. et al. (2014) Front. Immunol. 5:123.
  2. Hamann, J. et al. (1997) Immunogenetics 45:295.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Klimosch, S.N. et al. (2013) Blood 122:2380.

Long Name

Activation-Induced C-Type Lectin

Alternate Names

CLEC-2B, CLEC2B, CLECSF2, IFNRG1

Entrez Gene IDs

9976 (Human)

Gene Symbol

CLEC2B

UniProt

Q92478

Additional AICL/CLEC-2B Products

Product Documents for Human AICL/CLEC-2B Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human AICL/CLEC-2B Antibody

For research use only

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