Human Aldehyde Oxidase 1/AOX1 Antibody

Name: Human Aldehyde Oxidase 1/AOX1 Antibody
Brand: R&D Systems
SKU: AF5928

R&D Systems, part of Bio-Techne | Catalog # AF5928

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Aldehyde Oxidase 1/AOX1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Aldehyde Oxidase 1/AOX1
Asn302-Ser531
Accession # Q06278

Specificity

Detects human Aldehyde Oxidase 1/AOX1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Aldehyde Oxidase 1/AOX1 Antibody

Detection of Human Aldehyde Oxidase 1/AOX1 by Western Blot.

Western blot shows lysates of embryonic rat cortical neuron/glial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Aldehyde Oxidase 1/AOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Oxidase 1/AOX1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Aldehyde Oxidase 1/AOX1 in Human Liver.

Aldehyde Oxidase 1/AOX1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aldehyde Oxidase 1/AOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5928) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Aldehyde Oxidase 1/AOX1 in HepG2 Human Cell Line.

Aldehyde Oxidase 1/AOX1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Aldehyde Oxidase 1/AOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5928) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Aldehyde Oxidase 1/AOX1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: Embryonic rat cortical neuron/glial cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldehyde Oxidase 1/AOX1

AOX1 (Aldehyde oxidase 1; also AO and initially termed xanthine dehydrogenase/oxidase) is a 145‑150 kDa member of the large molybdo‑flavo family of enzymes. It is a homodimer widely expressed in cells such as hepatocytes, bronchial and intestinal epithelium and adrenal cortex. It is suggested to oxidize cyclic aldehydes to carboxylic acids and likely converts retinal to retinoic acid, gentistate aldehyde to gentisate and methylmalonate semialdehyde into methylmalonate. Human AOX1 is 1338 amino acids (aa) in length. It contains a 25 kDa N‑terminal region that possesses two Fe/S redox centers (aa 5‑162), a 40 kDa flavin‑containing domain (aa 236‑421), and an 85 kDa C‑terminal region that contains a molybdenum cofactor and substrate‑binding segment (aa 711‑1245). One isoform shows a two aa substitution for aa 354‑356. Over aa 302‑531, human AOX1 shares 82% aa identity with mouse AOX1.

Alternate Names

AOX1

Entrez Gene IDs

316 (Human); 11761 (Mouse)

Gene Symbol