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Human Allergin-1 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7638G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 767727

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Allergin-1
Met1-Lys227
Accession # Q7Z6M3

Specificity

Detects human Allergin-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Allergin-1 Alexa Fluor® 488-conjugated Antibody

Detection of Allergin-1 antibody in Human Blood-Derived Monocytes antibody by Flow Cytometry.

Detection of Allergin‑1 in Human Blood-Derived Monocytes by Flow Cytometry.

Human blood-derived monocytes were stained with Mouse Anti-Human Allergin-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7638G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Allergin-1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human blood-derived monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Allergin-1

Allergin-1, also known as MCA32 and c17orf60 is a 70-72 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils, dendritic cells, B cells and monocytes, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilonRI. Upon IgE mediated Fc epsilonRI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilonRI tetrameric complex, dampening IgE receptor downstream signaling and abrogating mast cell degranulation. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40-42 kDa splice variants exist that show deletions of one of the two Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 123-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. It would appear that mouse Allergin-1 exists in two forms; a soluble form lacking the TM segment, and a TM form that is analogous to the human 1S1 isoform (no two Ig-like domain-containing isoform exists). Within this context (aa 20-122 plus aa 218-227 in human), human and mouse share 48% aa sequence identity.

Long Name

Allergy Inhibitory Receptor 1

Alternate Names

Allergin1, C17orf60, MCA32, MCA32-1S2, MILR1

Entrez Gene IDs

284021 (Human); 380732 (Mouse)

Gene Symbol

MILR1

UniProt

Additional Allergin-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Allergin-1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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